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cell lines hek293t atcc catalog  (ATCC)


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    ATCC cell lines hek293t atcc catalog
    Cell Lines Hek293t Atcc Catalog, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 2806 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+lines+hek293t+atcc+catalog/TIBx%3B+Epithelial+liver%3B+Mouse/pm36029764-405-249-252
    Average 97 stars, based on 2806 article reviews
    cell lines hek293t atcc catalog - by Bioz Stars, 2026-11
    97/100 stars

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    Binding Assay:

    Article Title: Functional map of SARS-CoV-2 3CL protease reveals tolerant and immutable sites.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal GAL4 antibody (Clone RK5C1) Santa Cruz Biotechnology Catalog #sc-510, RRID: AB_627655 Anti-mouse IgG Kappa light chain binding protein HRP-conjugated Santa Cruz Biotechnology Catalog #sc-516102, RRID: AB_2687626 Bacterial and virus strains pBeloBAC11 bacterial artificial chromosome containing the SARS-Cov-2 genome with the ORF7a replaced with NanoLuc luciferase reporter, with the following mutations as independent constructs: WT, C145A, T25C, Q69C, C160F, P168R, H172Y, N180G, G258Q, T292M, V114W, L250G, L268N, E288F, D295G, or E166V Ye et al., 2020; this manuscript N/A Recombinant SARS-CoV-2 NanoLuc reporter viruses (with ORF7a deletion), with the following mutations as independent viruses: WT, C145A, T25C, Q69C, C160F, P168R, H172Y, N180G, G258Q, T292M, V114W, L250G, L268N, E288F, D295G, or E166V Ye et al., 2020; this manuscript N/A 10-beta Electrocompetent E. coli New England Biolabs Catalog #C3020K MDS42 E. coli Umenhoffer et al., 2010 N/A Chemicals, peptides, and recombinant proteins PF-07321332 (nirmatrelvir) Aobius Catalog #AOB14800 Remdesivir Selleckchem Catalog #S8932 Critical commercial assays Nucleobond Xtra Midi kit Macherey-Nagel Catalog #740410.50 Q5 High-Fidelity DNA Polymerase New England Biolabs Catalog #M0491L SuperSignal West Femto Maximum Sensitivity Substrate Thermo Fisher Scientific Catalog #34094 Nucleobond BAC 100 kit Takara Bio Catalog #740579 TRIzol Reagent Thermo Fisher Scientific Catalog #15596026 TURBO DNase Thermo Fisher Scientific Catalog #AM2238 TaqMan Fast Virus 1-Step Master Mix Thermo Fisher Scientific Catalog #4444432 TaqMan 2019nCoV Assay Kit v1 Thermo Fisher Scientific Catalog #A47532 Nano-Glo Luciferase Assay System Promega Catalog #N1130 Deposited data Raw sequencing data NCBI Short Read Archive BioProject ID PRJNA813935 Experimental models: Cell lines HEK293T ATCC Catalog #CRL-3216, RRID: CVCL_0063 Vero E6 ATCC Catalog #CRL-1586, RRID: CVCL_0574 Huh7-ACE2 Liu et al., 2022a N/A Experimental models: Organisms/strains Yeast, BY4741 strain (S288C background) Brachmann et al., 1998 N/A Yeast, PJ69-4a/ɑ strain James et al., 1996 N/A Oligonucleotides Oligos for mutagenizing SARS-CoV-2-3CL This manuscript Table S1 Oligos for amplifying SARS-CoV-2 3CL amplicons This manuscript Table S2 (Continued on next page) Cell Host & Microbe 30, 1354–1362.e1–e6, October 12, 2022 e1

    Virus:

    Article Title: Functional map of SARS-CoV-2 3CL protease reveals tolerant and immutable sites.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal GAL4 antibody (Clone RK5C1) Santa Cruz Biotechnology Catalog #sc-510, RRID: AB_627655 Anti-mouse IgG Kappa light chain binding protein HRP-conjugated Santa Cruz Biotechnology Catalog #sc-516102, RRID: AB_2687626 Bacterial and virus strains pBeloBAC11 bacterial artificial chromosome containing the SARS-Cov-2 genome with the ORF7a replaced with NanoLuc luciferase reporter, with the following mutations as independent constructs: WT, C145A, T25C, Q69C, C160F, P168R, H172Y, N180G, G258Q, T292M, V114W, L250G, L268N, E288F, D295G, or E166V Ye et al., 2020; this manuscript N/A Recombinant SARS-CoV-2 NanoLuc reporter viruses (with ORF7a deletion), with the following mutations as independent viruses: WT, C145A, T25C, Q69C, C160F, P168R, H172Y, N180G, G258Q, T292M, V114W, L250G, L268N, E288F, D295G, or E166V Ye et al., 2020; this manuscript N/A 10-beta Electrocompetent E. coli New England Biolabs Catalog #C3020K MDS42 E. coli Umenhoffer et al., 2010 N/A Chemicals, peptides, and recombinant proteins PF-07321332 (nirmatrelvir) Aobius Catalog #AOB14800 Remdesivir Selleckchem Catalog #S8932 Critical commercial assays Nucleobond Xtra Midi kit Macherey-Nagel Catalog #740410.50 Q5 High-Fidelity DNA Polymerase New England Biolabs Catalog #M0491L SuperSignal West Femto Maximum Sensitivity Substrate Thermo Fisher Scientific Catalog #34094 Nucleobond BAC 100 kit Takara Bio Catalog #740579 TRIzol Reagent Thermo Fisher Scientific Catalog #15596026 TURBO DNase Thermo Fisher Scientific Catalog #AM2238 TaqMan Fast Virus 1-Step Master Mix Thermo Fisher Scientific Catalog #4444432 TaqMan 2019nCoV Assay Kit v1 Thermo Fisher Scientific Catalog #A47532 Nano-Glo Luciferase Assay System Promega Catalog #N1130 Deposited data Raw sequencing data NCBI Short Read Archive BioProject ID PRJNA813935 Experimental models: Cell lines HEK293T ATCC Catalog #CRL-3216, RRID: CVCL_0063 Vero E6 ATCC Catalog #CRL-1586, RRID: CVCL_0574 Huh7-ACE2 Liu et al., 2022a N/A Experimental models: Organisms/strains Yeast, BY4741 strain (S288C background) Brachmann et al., 1998 N/A Yeast, PJ69-4a/ɑ strain James et al., 1996 N/A Oligonucleotides Oligos for mutagenizing SARS-CoV-2-3CL This manuscript Table S1 Oligos for amplifying SARS-CoV-2 3CL amplicons This manuscript Table S2 (Continued on next page) Cell Host & Microbe 30, 1354–1362.e1–e6, October 12, 2022 e1

    Luciferase:

    Article Title: Functional map of SARS-CoV-2 3CL protease reveals tolerant and immutable sites.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal GAL4 antibody (Clone RK5C1) Santa Cruz Biotechnology Catalog #sc-510, RRID: AB_627655 Anti-mouse IgG Kappa light chain binding protein HRP-conjugated Santa Cruz Biotechnology Catalog #sc-516102, RRID: AB_2687626 Bacterial and virus strains pBeloBAC11 bacterial artificial chromosome containing the SARS-Cov-2 genome with the ORF7a replaced with NanoLuc luciferase reporter, with the following mutations as independent constructs: WT, C145A, T25C, Q69C, C160F, P168R, H172Y, N180G, G258Q, T292M, V114W, L250G, L268N, E288F, D295G, or E166V Ye et al., 2020; this manuscript N/A Recombinant SARS-CoV-2 NanoLuc reporter viruses (with ORF7a deletion), with the following mutations as independent viruses: WT, C145A, T25C, Q69C, C160F, P168R, H172Y, N180G, G258Q, T292M, V114W, L250G, L268N, E288F, D295G, or E166V Ye et al., 2020; this manuscript N/A 10-beta Electrocompetent E. coli New England Biolabs Catalog #C3020K MDS42 E. coli Umenhoffer et al., 2010 N/A Chemicals, peptides, and recombinant proteins PF-07321332 (nirmatrelvir) Aobius Catalog #AOB14800 Remdesivir Selleckchem Catalog #S8932 Critical commercial assays Nucleobond Xtra Midi kit Macherey-Nagel Catalog #740410.50 Q5 High-Fidelity DNA Polymerase New England Biolabs Catalog #M0491L SuperSignal West Femto Maximum Sensitivity Substrate Thermo Fisher Scientific Catalog #34094 Nucleobond BAC 100 kit Takara Bio Catalog #740579 TRIzol Reagent Thermo Fisher Scientific Catalog #15596026 TURBO DNase Thermo Fisher Scientific Catalog #AM2238 TaqMan Fast Virus 1-Step Master Mix Thermo Fisher Scientific Catalog #4444432 TaqMan 2019nCoV Assay Kit v1 Thermo Fisher Scientific Catalog #A47532 Nano-Glo Luciferase Assay System Promega Catalog #N1130 Deposited data Raw sequencing data NCBI Short Read Archive BioProject ID PRJNA813935 Experimental models: Cell lines HEK293T ATCC Catalog #CRL-3216, RRID: CVCL_0063 Vero E6 ATCC Catalog #CRL-1586, RRID: CVCL_0574 Huh7-ACE2 Liu et al., 2022a N/A Experimental models: Organisms/strains Yeast, BY4741 strain (S288C background) Brachmann et al., 1998 N/A Yeast, PJ69-4a/ɑ strain James et al., 1996 N/A Oligonucleotides Oligos for mutagenizing SARS-CoV-2-3CL This manuscript Table S1 Oligos for amplifying SARS-CoV-2 3CL amplicons This manuscript Table S2 (Continued on next page) Cell Host & Microbe 30, 1354–1362.e1–e6, October 12, 2022 e1

    Construct:

    Article Title: Functional map of SARS-CoV-2 3CL protease reveals tolerant and immutable sites.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal GAL4 antibody (Clone RK5C1) Santa Cruz Biotechnology Catalog #sc-510, RRID: AB_627655 Anti-mouse IgG Kappa light chain binding protein HRP-conjugated Santa Cruz Biotechnology Catalog #sc-516102, RRID: AB_2687626 Bacterial and virus strains pBeloBAC11 bacterial artificial chromosome containing the SARS-Cov-2 genome with the ORF7a replaced with NanoLuc luciferase reporter, with the following mutations as independent constructs: WT, C145A, T25C, Q69C, C160F, P168R, H172Y, N180G, G258Q, T292M, V114W, L250G, L268N, E288F, D295G, or E166V Ye et al., 2020; this manuscript N/A Recombinant SARS-CoV-2 NanoLuc reporter viruses (with ORF7a deletion), with the following mutations as independent viruses: WT, C145A, T25C, Q69C, C160F, P168R, H172Y, N180G, G258Q, T292M, V114W, L250G, L268N, E288F, D295G, or E166V Ye et al., 2020; this manuscript N/A 10-beta Electrocompetent E. coli New England Biolabs Catalog #C3020K MDS42 E. coli Umenhoffer et al., 2010 N/A Chemicals, peptides, and recombinant proteins PF-07321332 (nirmatrelvir) Aobius Catalog #AOB14800 Remdesivir Selleckchem Catalog #S8932 Critical commercial assays Nucleobond Xtra Midi kit Macherey-Nagel Catalog #740410.50 Q5 High-Fidelity DNA Polymerase New England Biolabs Catalog #M0491L SuperSignal West Femto Maximum Sensitivity Substrate Thermo Fisher Scientific Catalog #34094 Nucleobond BAC 100 kit Takara Bio Catalog #740579 TRIzol Reagent Thermo Fisher Scientific Catalog #15596026 TURBO DNase Thermo Fisher Scientific Catalog #AM2238 TaqMan Fast Virus 1-Step Master Mix Thermo Fisher Scientific Catalog #4444432 TaqMan 2019nCoV Assay Kit v1 Thermo Fisher Scientific Catalog #A47532 Nano-Glo Luciferase Assay System Promega Catalog #N1130 Deposited data Raw sequencing data NCBI Short Read Archive BioProject ID PRJNA813935 Experimental models: Cell lines HEK293T ATCC Catalog #CRL-3216, RRID: CVCL_0063 Vero E6 ATCC Catalog #CRL-1586, RRID: CVCL_0574 Huh7-ACE2 Liu et al., 2022a N/A Experimental models: Organisms/strains Yeast, BY4741 strain (S288C background) Brachmann et al., 1998 N/A Yeast, PJ69-4a/ɑ strain James et al., 1996 N/A Oligonucleotides Oligos for mutagenizing SARS-CoV-2-3CL This manuscript Table S1 Oligos for amplifying SARS-CoV-2 3CL amplicons This manuscript Table S2 (Continued on next page) Cell Host & Microbe 30, 1354–1362.e1–e6, October 12, 2022 e1

    Recombinant:

    Article Title: Functional map of SARS-CoV-2 3CL protease reveals tolerant and immutable sites.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal GAL4 antibody (Clone RK5C1) Santa Cruz Biotechnology Catalog #sc-510, RRID: AB_627655 Anti-mouse IgG Kappa light chain binding protein HRP-conjugated Santa Cruz Biotechnology Catalog #sc-516102, RRID: AB_2687626 Bacterial and virus strains pBeloBAC11 bacterial artificial chromosome containing the SARS-Cov-2 genome with the ORF7a replaced with NanoLuc luciferase reporter, with the following mutations as independent constructs: WT, C145A, T25C, Q69C, C160F, P168R, H172Y, N180G, G258Q, T292M, V114W, L250G, L268N, E288F, D295G, or E166V Ye et al., 2020; this manuscript N/A Recombinant SARS-CoV-2 NanoLuc reporter viruses (with ORF7a deletion), with the following mutations as independent viruses: WT, C145A, T25C, Q69C, C160F, P168R, H172Y, N180G, G258Q, T292M, V114W, L250G, L268N, E288F, D295G, or E166V Ye et al., 2020; this manuscript N/A 10-beta Electrocompetent E. coli New England Biolabs Catalog #C3020K MDS42 E. coli Umenhoffer et al., 2010 N/A Chemicals, peptides, and recombinant proteins PF-07321332 (nirmatrelvir) Aobius Catalog #AOB14800 Remdesivir Selleckchem Catalog #S8932 Critical commercial assays Nucleobond Xtra Midi kit Macherey-Nagel Catalog #740410.50 Q5 High-Fidelity DNA Polymerase New England Biolabs Catalog #M0491L SuperSignal West Femto Maximum Sensitivity Substrate Thermo Fisher Scientific Catalog #34094 Nucleobond BAC 100 kit Takara Bio Catalog #740579 TRIzol Reagent Thermo Fisher Scientific Catalog #15596026 TURBO DNase Thermo Fisher Scientific Catalog #AM2238 TaqMan Fast Virus 1-Step Master Mix Thermo Fisher Scientific Catalog #4444432 TaqMan 2019nCoV Assay Kit v1 Thermo Fisher Scientific Catalog #A47532 Nano-Glo Luciferase Assay System Promega Catalog #N1130 Deposited data Raw sequencing data NCBI Short Read Archive BioProject ID PRJNA813935 Experimental models: Cell lines HEK293T ATCC Catalog #CRL-3216, RRID: CVCL_0063 Vero E6 ATCC Catalog #CRL-1586, RRID: CVCL_0574 Huh7-ACE2 Liu et al., 2022a N/A Experimental models: Organisms/strains Yeast, BY4741 strain (S288C background) Brachmann et al., 1998 N/A Yeast, PJ69-4a/ɑ strain James et al., 1996 N/A Oligonucleotides Oligos for mutagenizing SARS-CoV-2-3CL This manuscript Table S1 Oligos for amplifying SARS-CoV-2 3CL amplicons This manuscript Table S2 (Continued on next page) Cell Host & Microbe 30, 1354–1362.e1–e6, October 12, 2022 e1

    BAC Assay:

    Article Title: Functional map of SARS-CoV-2 3CL protease reveals tolerant and immutable sites.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal GAL4 antibody (Clone RK5C1) Santa Cruz Biotechnology Catalog #sc-510, RRID: AB_627655 Anti-mouse IgG Kappa light chain binding protein HRP-conjugated Santa Cruz Biotechnology Catalog #sc-516102, RRID: AB_2687626 Bacterial and virus strains pBeloBAC11 bacterial artificial chromosome containing the SARS-Cov-2 genome with the ORF7a replaced with NanoLuc luciferase reporter, with the following mutations as independent constructs: WT, C145A, T25C, Q69C, C160F, P168R, H172Y, N180G, G258Q, T292M, V114W, L250G, L268N, E288F, D295G, or E166V Ye et al., 2020; this manuscript N/A Recombinant SARS-CoV-2 NanoLuc reporter viruses (with ORF7a deletion), with the following mutations as independent viruses: WT, C145A, T25C, Q69C, C160F, P168R, H172Y, N180G, G258Q, T292M, V114W, L250G, L268N, E288F, D295G, or E166V Ye et al., 2020; this manuscript N/A 10-beta Electrocompetent E. coli New England Biolabs Catalog #C3020K MDS42 E. coli Umenhoffer et al., 2010 N/A Chemicals, peptides, and recombinant proteins PF-07321332 (nirmatrelvir) Aobius Catalog #AOB14800 Remdesivir Selleckchem Catalog #S8932 Critical commercial assays Nucleobond Xtra Midi kit Macherey-Nagel Catalog #740410.50 Q5 High-Fidelity DNA Polymerase New England Biolabs Catalog #M0491L SuperSignal West Femto Maximum Sensitivity Substrate Thermo Fisher Scientific Catalog #34094 Nucleobond BAC 100 kit Takara Bio Catalog #740579 TRIzol Reagent Thermo Fisher Scientific Catalog #15596026 TURBO DNase Thermo Fisher Scientific Catalog #AM2238 TaqMan Fast Virus 1-Step Master Mix Thermo Fisher Scientific Catalog #4444432 TaqMan 2019nCoV Assay Kit v1 Thermo Fisher Scientific Catalog #A47532 Nano-Glo Luciferase Assay System Promega Catalog #N1130 Deposited data Raw sequencing data NCBI Short Read Archive BioProject ID PRJNA813935 Experimental models: Cell lines HEK293T ATCC Catalog #CRL-3216, RRID: CVCL_0063 Vero E6 ATCC Catalog #CRL-1586, RRID: CVCL_0574 Huh7-ACE2 Liu et al., 2022a N/A Experimental models: Organisms/strains Yeast, BY4741 strain (S288C background) Brachmann et al., 1998 N/A Yeast, PJ69-4a/ɑ strain James et al., 1996 N/A Oligonucleotides Oligos for mutagenizing SARS-CoV-2-3CL This manuscript Table S1 Oligos for amplifying SARS-CoV-2 3CL amplicons This manuscript Table S2 (Continued on next page) Cell Host & Microbe 30, 1354–1362.e1–e6, October 12, 2022 e1

    Sequencing:

    Article Title: Functional map of SARS-CoV-2 3CL protease reveals tolerant and immutable sites.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal GAL4 antibody (Clone RK5C1) Santa Cruz Biotechnology Catalog #sc-510, RRID: AB_627655 Anti-mouse IgG Kappa light chain binding protein HRP-conjugated Santa Cruz Biotechnology Catalog #sc-516102, RRID: AB_2687626 Bacterial and virus strains pBeloBAC11 bacterial artificial chromosome containing the SARS-Cov-2 genome with the ORF7a replaced with NanoLuc luciferase reporter, with the following mutations as independent constructs: WT, C145A, T25C, Q69C, C160F, P168R, H172Y, N180G, G258Q, T292M, V114W, L250G, L268N, E288F, D295G, or E166V Ye et al., 2020; this manuscript N/A Recombinant SARS-CoV-2 NanoLuc reporter viruses (with ORF7a deletion), with the following mutations as independent viruses: WT, C145A, T25C, Q69C, C160F, P168R, H172Y, N180G, G258Q, T292M, V114W, L250G, L268N, E288F, D295G, or E166V Ye et al., 2020; this manuscript N/A 10-beta Electrocompetent E. coli New England Biolabs Catalog #C3020K MDS42 E. coli Umenhoffer et al., 2010 N/A Chemicals, peptides, and recombinant proteins PF-07321332 (nirmatrelvir) Aobius Catalog #AOB14800 Remdesivir Selleckchem Catalog #S8932 Critical commercial assays Nucleobond Xtra Midi kit Macherey-Nagel Catalog #740410.50 Q5 High-Fidelity DNA Polymerase New England Biolabs Catalog #M0491L SuperSignal West Femto Maximum Sensitivity Substrate Thermo Fisher Scientific Catalog #34094 Nucleobond BAC 100 kit Takara Bio Catalog #740579 TRIzol Reagent Thermo Fisher Scientific Catalog #15596026 TURBO DNase Thermo Fisher Scientific Catalog #AM2238 TaqMan Fast Virus 1-Step Master Mix Thermo Fisher Scientific Catalog #4444432 TaqMan 2019nCoV Assay Kit v1 Thermo Fisher Scientific Catalog #A47532 Nano-Glo Luciferase Assay System Promega Catalog #N1130 Deposited data Raw sequencing data NCBI Short Read Archive BioProject ID PRJNA813935 Experimental models: Cell lines HEK293T ATCC Catalog #CRL-3216, RRID: CVCL_0063 Vero E6 ATCC Catalog #CRL-1586, RRID: CVCL_0574 Huh7-ACE2 Liu et al., 2022a N/A Experimental models: Organisms/strains Yeast, BY4741 strain (S288C background) Brachmann et al., 1998 N/A Yeast, PJ69-4a/ɑ strain James et al., 1996 N/A Oligonucleotides Oligos for mutagenizing SARS-CoV-2-3CL This manuscript Table S1 Oligos for amplifying SARS-CoV-2 3CL amplicons This manuscript Table S2 (Continued on next page) Cell Host & Microbe 30, 1354–1362.e1–e6, October 12, 2022 e1



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    cell lines hek293t atcc catalog  (ATCC)
    97
    ATCC cell lines hek293t atcc catalog
    Cell Lines Hek293t Atcc Catalog, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+lines+hek293t+atcc+catalog/TIBx%3B+Epithelial+liver%3B+Mouse/pm36029764-405-249-252
    Average 97 stars, based on 1 article reviews
    cell lines hek293t atcc catalog - by Bioz Stars, 2026-11
    97/100 stars
    		  Buy from Supplier

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